Have questions? We may already have the answers. Take a look at our Frequently Asked Questions. If you have more questions please Contact Us?
- Preparation of the bioink: Living cells are mixed with biomaterials such as hydrogels, which provide structural support and a suitable environment for the cells to grow and differentiate. The bioink should have the right consistency and properties to be extruded through the printing nozzle.
- Design of the 3D model: A digital 3D model of the desired tissue or organ is created using specialized software.
- Printing: The bioink is loaded into a syringe or other extrusion device and is deposited layer-by-layer onto a substrate or scaffold according to the digital model. The extrusion nozzle moves in a pre-defined pattern, releasing the bioink as it moves.
- Crosslinking or solidification: Once the desired structure is printed, the bioink may need to be crosslinked or solidified to maintain its shape and stability. This can be done by various methods such as exposure to UV light, temperature, or chemicals.
- Cell culture and maturation: The printed structure is placed in an incubator or other suitable environment for the cells to grow, differentiate, and mature. Over time, the tissue may develop more complex properties and functions, depending on the type of cells and biomaterials used.
Overall, extrusion-based bioprinting is a versatile and widely used method of creating 3D structures of living tissues and organs.